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DDX5/p68 RNA Helicase Recombinant Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61644r-hrp-100uL
Product Name DDX5/p68 RNA Helicase Recombinant Antibody, HRP Conjugated
Applications
WB IHC-P IHC-F
Reactivity Human, Mouse, Rat
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DDX5
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1655
Swiss Prot P17844
Subcellular location Nucleus
Synonyms Ddx5; G17P1; HELR; HLR1; HUMP68; p68 RNA helicase; RNA helicase p68.
Background Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is DDX5/p68 RNA Helicase Recombinant Antibody, HRP Conjugated (bsm-61644R-HRP) validated for, and at what dilution?

bsm-61644R-HRP is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DDX5/p68 RNA Helicase Recombinant Antibody, HRP Conjugated (bsm-61644R-HRP) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61644R-HRP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DDX5/p68 RNA Helicase Recombinant Antibody, HRP Conjugated (bsm-61644R-HRP)?

KLH conjugated synthetic peptide derived from human DDX5