TRADD Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with TRADD monoclonal antibody, unconjugated (bsm-61653R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with TRADD monoclonal antibody, unconjugated (bsm-61653R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IP
ICC/IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-61653r-TR
Product Name	TRADD Recombinant Antibody
Applications	WB, IP, ICC/IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TRADD
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8717
Swiss Prot	Q15628
Subcellular location	Cytoplasm, Nucleus
Synonyms	AA930854; TNFR1 associated DEATH domain protein; tradd.
Background	Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD.Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
Application Dilution
WB	1:300-5000
IP	1-2ug
ICC/IF	1:50-100