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RAP80 Recombinant Antibody, PE-Cy7 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-61657r-pe-cy7
Product Name RAP80 Recombinant Antibody, PE-Cy7 Conjugated
Applications
WB FCM
Reactivity Human
Specifications
Conjugation PE-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RAP80
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51720
Swiss Prot Q96RL1
Synonyms BRCA1-A complex subunit RAP80; RAP80; Receptor associated protein 80; RIP110; Rxrip110; UIMC1; X2HRIP110.
Background Ubiquitin-binding protein.Specifically recognizes and binds 'Lys-63'-linked ubiquitin.Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs).
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is RAP80 Recombinant Antibody, PE-Cy7 Conjugated (bsm-61657R-PE-Cy7) validated for, and at what dilution?

bsm-61657R-PE-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RAP80 Recombinant Antibody, PE-Cy7 Conjugated (bsm-61657R-PE-Cy7) react with?

Tested reactivity: Human.

How should bsm-61657R-PE-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RAP80 Recombinant Antibody, PE-Cy7 Conjugated (bsm-61657R-PE-Cy7)?

KLH conjugated synthetic peptide derived from human RAP80

What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bsm-61657R-PE-Cy7)?

PE-Cy7 on bsm-61657R-PE-Cy7: excitation / emission 488nm/780nm.