RAP80 Recombinant Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61657r-percp |
| Product Name | RAP80 Recombinant Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAP80 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51720 |
| Swiss Prot | Q96RL1 |
| Synonyms | BRCA1-A complex subunit RAP80; RAP80; Receptor associated protein 80; RIP110; Rxrip110; UIMC1; X2HRIP110. |
| Background | Ubiquitin-binding protein.Specifically recognizes and binds 'Lys-63'-linked ubiquitin.Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is RAP80 Recombinant Antibody, PerCP Conjugated (bsm-61657R-PerCP) validated for, and at what dilution?
bsm-61657R-PerCP is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAP80 Recombinant Antibody, PerCP Conjugated (bsm-61657R-PerCP) react with?
Tested reactivity: Human.
How should bsm-61657R-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAP80 Recombinant Antibody, PerCP Conjugated (bsm-61657R-PerCP)?
KLH conjugated synthetic peptide derived from human RAP80
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61657R-PerCP)?
PerCP on bsm-61657R-PerCP: excitation / emission 482nm/677nm.