SKP1 Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61662r-bf680 |
| Product Name | SKP1 Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SKP1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6500 |
| Swiss Prot | P63208 |
| Subcellular location | Extracellular, Cytoplasm, Nucleus, Secreted |
| Synonyms | OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L. |
| Background | Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is SKP1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61662R-BF680) validated for, and at what dilution?
bsm-61662R-BF680 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SKP1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61662R-BF680) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61662R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SKP1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61662R-BF680)?
KLH conjugated synthetic peptide derived from human SKP1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-61662R-BF680)?
AbBy Fluor-680 on bsm-61662R-BF680: excitation / emission 679nm/702nm.