macro H2A.1 Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61665r-apc |
| Product Name | macro H2A.1 Recombinant Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Histone mH2A1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9555 |
| Swiss Prot | O75367 |
| Subcellular location | Nucleus |
| Synonyms | H2A.y; H2A/y; H2AF12M; H2AFJ; H2afy; Histone H2A.Y; Histone macroH2A1; Histone macroH2A1.1; Histone macroH2A1.2; mH2a;mH2A1. |
| Background | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription.Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:200-500 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is macro H2A.1 Recombinant Antibody, APC Conjugated (bsm-61665R-APC) validated for, and at what dilution?
bsm-61665R-APC is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:200-500); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does macro H2A.1 Recombinant Antibody, APC Conjugated (bsm-61665R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61665R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise macro H2A.1 Recombinant Antibody, APC Conjugated (bsm-61665R-APC)?
KLH conjugated synthetic peptide derived from human Histone mH2A1
What are the excitation and emission wavelengths of the APC conjugate (bsm-61665R-APC)?
APC on bsm-61665R-APC: excitation / emission 650nm/660nm.