PAK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61673r-pe-cy3 |
| Product Name | PAK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PAK2 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5062 |
| Swiss Prot | Q13177 |
| Subcellular location | Cytoplasm |
| Synonyms | CB422; Gamma PAK; hPAK65; p27; p34; p58; p65PAK; PAK-2p34; Pak2; PAK65; PAKgamma; S6 H4 kinase. |
| Background | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation.Acts as a downstream effector of the small GTPases CDC42 and RAC1.Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PAK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61673R-PE-Cy3) validated for, and at what dilution?
bsm-61673R-PE-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PAK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61673R-PE-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61673R-PE-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PAK2 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61673R-PE-Cy3)?
KLH conjugated synthetic peptide derived from human PAK2
What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-61673R-PE-Cy3)?
PE-Cy3 on bsm-61673R-PE-Cy3: excitation / emission 488nmn/578nm.