SHC1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61677r-pe-cy3 |
| Product Name | SHC1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SHC1 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6464 |
| Swiss Prot | P29353 |
| Subcellular location | Cytoplasm |
| Synonyms | SH2 domain protein C1; SHC; SHC-transforming protein 1; SHCA; Src homology 2 domain-containing-transforming protein C1. |
| Background | Shc1 IS an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Three isoforms(p66, p52 and p46), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis and apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is SHC1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61677R-PE-Cy3) validated for, and at what dilution?
bsm-61677R-PE-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SHC1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61677R-PE-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61677R-PE-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SHC1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61677R-PE-Cy3)?
KLH conjugated synthetic peptide derived from human SHC1
What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-61677R-PE-Cy3)?
PE-Cy3 on bsm-61677R-PE-Cy3: excitation / emission 488nmn/578nm.