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CD166 Recombinant Antibody, FITC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61678r-fitc
Product Name CD166 Recombinant Antibody, FITC Conjugated
Applications
WB FCM IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ALCAM
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 214
Swiss Prot Q13740
Subcellular location Cell membrane
Synonyms ALCAM; CD166; MEMD.
Background Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is CD166 Recombinant Antibody, FITC Conjugated (bsm-61678R-FITC) validated for, and at what dilution?

bsm-61678R-FITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD166 Recombinant Antibody, FITC Conjugated (bsm-61678R-FITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61678R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD166 Recombinant Antibody, FITC Conjugated (bsm-61678R-FITC)?

KLH conjugated synthetic peptide derived from human ALCAM

What are the excitation and emission wavelengths of the FITC conjugate (bsm-61678R-FITC)?

FITC on bsm-61678R-FITC: excitation / emission 494nm/518nm.