SMYD3 Recombinant Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate. Using SMYD3 (bsm-61686R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate. Using SMYD3 (bsm-61686R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IP
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61686r
Product Name	SMYD3 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SMYD3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	64754
Swiss Prot	Q9H7B4
Synonyms	KMT3E; Smyd3; ZMYND1; ZNFN3A1.
Background	Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation.Also methylates 'Lys-5' of histone H4.Plays an important role in transcriptional activation as a member of an RNA polymerase complex.
Application Dilution
WB	1:300-5000
IHC-P	FCM1:20-100
IHC-F	IHC-P1:200-400
IP	IHC-F1:100-500
IF	ICC1:50-200
Flow-Cyt	IP1-2ug
ICC/IF	IF