EGR1 Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61687r-bf555 |
| Product Name | EGR1 Recombinant Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EGR 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1958 |
| Swiss Prot | P18146 |
| Subcellular location | Nucleus |
| Synonyms | TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; EGR1. |
| Background | Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is EGR1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61687R-BF555) validated for, and at what dilution?
bsm-61687R-BF555 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EGR1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61687R-BF555) react with?
Tested reactivity: Human.
How should bsm-61687R-BF555 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EGR1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61687R-BF555)?
KLH conjugated synthetic peptide derived from human EGR 1
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-61687R-BF555)?
AbBy Fluor-555 on bsm-61687R-BF555: excitation / emission 553nm/568nm.