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EGR1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-61687r-pe-cy3
Product Name EGR1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation PE-Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EGR 1
Clonality Monoclonal
Clone # Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1958
Swiss Prot P18146
Subcellular location Nucleus
Synonyms TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; EGR1.
Background Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is EGR1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61687R-PE-Cy3) validated for, and at what dilution?

bsm-61687R-PE-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EGR1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61687R-PE-Cy3) react with?

Tested reactivity: Human.

How should bsm-61687R-PE-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise EGR1 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61687R-PE-Cy3)?

KLH conjugated synthetic peptide derived from human EGR 1

What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-61687R-PE-Cy3)?

PE-Cy3 on bsm-61687R-PE-Cy3: excitation / emission 488nmn/578nm.