ELAVL1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with ELAVL1 monoclonal antibody, unconjugated (bsm-61688R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ELAVL1 monoclonal antibody, unconjugated (bsm-61688R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Heart\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Heart\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Heart\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Heart\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Heart\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Heart\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer\uff1b Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ELAVL1 Monoclonal Antibody, Unconjugated (bsm-61688R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IP
IF
Flow-Cyt
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61688r
Product Name	ELAVL1 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ELAVL1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1994
Swiss Prot	Q15717
Subcellular location	Cytoplasm, Nucleus
Synonyms	HUR; Hua; MelG; ELAV1.
Background	RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability.Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	1-2ug
IF
Flow-Cyt	1ug/Test
ICC/IF	1:50-100