Cullin 1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61690r-apc-cy5 |
| Product Name | Cullin 1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cullin 1 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8454 |
| Swiss Prot | Q13616 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CUL1; Cullin1. |
| Background | Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Cullin 1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61690R-APC-Cy5) validated for, and at what dilution?
bsm-61690R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cullin 1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61690R-APC-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61690R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cullin 1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61690R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human Cullin 1
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-61690R-APC-Cy5)?
APC-Cy5 on bsm-61690R-APC-Cy5: excitation / emission 650nm/670nm.