KSR1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61694r-apc-cy5 |
| Product Name | KSR1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KSR1 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8844 |
| Swiss Prot | Q8IVT5 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | BKSR1; dKsr; EK31; KSR; KSR1; RSU2; SR31. |
| Background | Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases.Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KSR1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61694R-APC-Cy5) validated for, and at what dilution?
bsm-61694R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does KSR1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61694R-APC-Cy5) react with?
Tested reactivity: Human, Mouse.
How should bsm-61694R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KSR1 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61694R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human KSR1
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-61694R-APC-Cy5)?
APC-Cy5 on bsm-61694R-APC-Cy5: excitation / emission 650nm/670nm.