PDK2 Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61695r-cy7 |
| Product Name | PDK2 Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDK2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5164 |
| Swiss Prot | Q15119 |
| Subcellular location | Cytoplasm |
| Synonyms | PDHK2; PDK2. |
| Background | Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PDK2 Recombinant Antibody, Cy7 Conjugated (bsm-61695R-Cy7) validated for, and at what dilution?
bsm-61695R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDK2 Recombinant Antibody, Cy7 Conjugated (bsm-61695R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61695R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDK2 Recombinant Antibody, Cy7 Conjugated (bsm-61695R-Cy7)?
KLH conjugated synthetic peptide derived from human PDK2
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-61695R-Cy7)?
Cy7 on bsm-61695R-Cy7: excitation / emission 743nm/767nm.