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PDHB Recombinant Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61702r-bf350
Product Name PDHB Recombinant Antibody, AbBy Fluor-350 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PDHB
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5162
Swiss Prot P11177
Subcellular location Cytoplasm
Synonyms pdhB; DHBD; PDHE1 B; PDHE1-B; PHE1B.
Background The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is PDHB Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61702R-BF350) validated for, and at what dilution?

bsm-61702R-BF350 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PDHB Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61702R-BF350) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61702R-BF350 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PDHB Recombinant Antibody, AbBy Fluor-350 Conjugated (bsm-61702R-BF350)?

KLH conjugated synthetic peptide derived from human PDHB

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-61702R-BF350)?

AbBy Fluor-350 on bsm-61702R-BF350: excitation / emission 346nm/442nm.