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RRM2 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with RRM2 monoclonal antibody, unconjugated (bsm-61703R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with RRM2 monoclonal antibody, unconjugated (bsm-61703R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed PANC-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RRM2) monoclonal Antibody, unconjugated (bsm-61703R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed PANC-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RRM2) monoclonal Antibody, unconjugated (bsm-61703R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • WB
  • IF(ICC)
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-61703r
Product Name RRM2 Recombinant Antibody
Applications
WB IF(ICC) IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RRM2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6241
Swiss Prot P31350
Subcellular location Cell membrane
Synonyms R2;RR2M.
Background Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
Application Dilution
WB 1:500-2000
IF(ICC) IFICC1:50-200
IP IP1:20-50

Frequently asked questions

Which applications is RRM2 Recombinant Antibody (bsm-61703R) validated for, and at what dilution?

bsm-61703R is validated for WB(WB) (1:500-2000); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RRM2 Recombinant Antibody (bsm-61703R) react with?

Tested reactivity: Human.

How should bsm-61703R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise RRM2 Recombinant Antibody (bsm-61703R)?

KLH conjugated synthetic peptide derived from human RRM2

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