RRM2 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with RRM2 monoclonal antibody, unconjugated (bsm-61703R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with RRM2 monoclonal antibody, unconjugated (bsm-61703R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$4% Paraformaldehyde-fixed PANC-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RRM2) monoclonal Antibody, unconjugated (bsm-61703R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed PANC-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RRM2) monoclonal Antibody, unconjugated (bsm-61703R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

WB
IF(ICC)
IP

Reactivity

Human

Overview
Catalog #	bsm-61703r-100ul
Product Name	RRM2 Recombinant Antibody
Applications	WB, IF(ICC), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RRM2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6241
Swiss Prot	P31350
Subcellular location	Cell membrane
Synonyms	R2;RR2M.
Background	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200
IP	IP1:20-50