SAE2 / UBA2 Recombinant Antibody

$Western blot analysis of A549 cell lysate. Using SAE2 \/ UBA2 (bsm-61707R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of A549 cell lysate. Using SAE2 \/ UBA2 (bsm-61707R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
IHC-P
IHC-F
IP
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61707r
Product Name	SAE2 / UBA2 Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IP, IF, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human UBA2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10054
Swiss Prot	Q9UBT2
Synonyms	ARX; SAE2; UBA2; UBLE1B.
Background	The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IP	IFICC1:50-200
IF	IP1-2ug
ICC/IF	IF