beta I Tubulin Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61710r-rbitc |
| Product Name | beta I Tubulin Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Monkey |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human beta I Tubulin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81027 |
| Swiss Prot | Q9H4B7 |
| Subcellular location | Cytoplasm |
| Synonyms | Beta tubulin 1, class VI;Class VI beta tubulin antibody;TBB1_HUMAN;TUBB1;Tubulin beta 1 class VI;Tubulin, beta 1 antibody. |
| Background | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Application Dilution | |
| WB | 1:500-5000 |
| FCM | FCM1:50-200 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is beta I Tubulin Recombinant Antibody, RBITC Conjugated (bsm-61710R-RBITC) validated for, and at what dilution?
bsm-61710R-RBITC is validated for WB(WB) (1:500-5000); FCM(FCM) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does beta I Tubulin Recombinant Antibody, RBITC Conjugated (bsm-61710R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Monkey.
How should bsm-61710R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise beta I Tubulin Recombinant Antibody, RBITC Conjugated (bsm-61710R-RBITC)?
KLH conjugated synthetic peptide derived from human beta I Tubulin
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61710R-RBITC)?
RBITC on bsm-61710R-RBITC: excitation / emission 570nm/595nm.