BAF53A Recombinant Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61726r-fitc |
| Product Name | BAF53A Recombinant Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ACTL6A |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 86 |
| Swiss Prot | O96019 |
| Subcellular location | Nucleus |
| Synonyms | ACTL6; ACTL6A; Arp4; ArpNbeta; BAF53; BAF53A; hArpN beta; INO80K. |
| Background | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is BAF53A Recombinant Antibody, FITC Conjugated (bsm-61726R-FITC) validated for, and at what dilution?
bsm-61726R-FITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BAF53A Recombinant Antibody, FITC Conjugated (bsm-61726R-FITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61726R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BAF53A Recombinant Antibody, FITC Conjugated (bsm-61726R-FITC)?
KLH conjugated synthetic peptide derived from human ACTL6A
What are the excitation and emission wavelengths of the FITC conjugate (bsm-61726R-FITC)?
FITC on bsm-61726R-FITC: excitation / emission 494nm/518nm.