CYP1A2 Recombinant Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61730r-fitc |
| Product Name | CYP1A2 Recombinant Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cytochrome P450 1A2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1544 |
| Swiss Prot | P05177 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CP12; CYP1A2; CYPIA2; P3 450; P450 4; P450 P3. |
| Background | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is CYP1A2 Recombinant Antibody, FITC Conjugated (bsm-61730R-FITC) validated for, and at what dilution?
bsm-61730R-FITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP1A2 Recombinant Antibody, FITC Conjugated (bsm-61730R-FITC) react with?
Tested reactivity: Human.
How should bsm-61730R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP1A2 Recombinant Antibody, FITC Conjugated (bsm-61730R-FITC)?
KLH conjugated synthetic peptide derived from human Cytochrome P450 1A2
What are the excitation and emission wavelengths of the FITC conjugate (bsm-61730R-FITC)?
FITC on bsm-61730R-FITC: excitation / emission 494nm/518nm.