Endo G Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61741r-cy3 |
| Product Name | Endo G Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Endo G |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2021 |
| Swiss Prot | Q14249 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | EndoG; EndonucleaseG; Mitochondrial endonuclease G; NUCG_HUMAN. |
| Background | Endo G is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is Endo G Recombinant Antibody, Cy3 Conjugated (bsm-61741R-Cy3) validated for, and at what dilution?
bsm-61741R-Cy3 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Endo G Recombinant Antibody, Cy3 Conjugated (bsm-61741R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bsm-61741R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Endo G Recombinant Antibody, Cy3 Conjugated (bsm-61741R-Cy3)?
KLH conjugated synthetic peptide derived from human Endo G
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61741R-Cy3)?
Cy3 on bsm-61741R-Cy3: excitation / emission 512,550nm/570,615nm.