Geminin Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61750r-bf488 |
| Product Name | Geminin Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Geminin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51053 |
| Swiss Prot | O75496 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Gem; Geminin; GMNN. |
| Background | Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Geminin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61750R-BF488) validated for, and at what dilution?
bsm-61750R-BF488 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Geminin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61750R-BF488) react with?
Tested reactivity: Human.
How should bsm-61750R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Geminin Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61750R-BF488)?
KLH conjugated synthetic peptide derived from human Geminin
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61750R-BF488)?
AbBy Fluor-488 on bsm-61750R-BF488: excitation / emission 499nm/519nm.