PHOX2B Recombinant Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61755r-fitc |
| Product Name | PHOX2B Recombinant Antibody, FITC Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PHOX2B |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8929 |
| Swiss Prot | Q99453 |
| Subcellular location | Nucleus |
| Synonyms | NBLST2; NBPhox; PHOX 2B; PHOX2B; PMX 2B; PMX2B. |
| Background | Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PHOX2B Recombinant Antibody, FITC Conjugated (bsm-61755R-FITC) validated for, and at what dilution?
bsm-61755R-FITC is validated for WB(WB) (1:500-2000); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PHOX2B Recombinant Antibody, FITC Conjugated (bsm-61755R-FITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bsm-61755R-FITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PHOX2B Recombinant Antibody, FITC Conjugated (bsm-61755R-FITC)?
KLH conjugated synthetic peptide derived from human PHOX2B
What are the excitation and emission wavelengths of the FITC conjugate (bsm-61755R-FITC)?
FITC on bsm-61755R-FITC: excitation / emission 494nm/518nm.