PHOX2B Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with PHOX2B monoclonal antibody, unconjugated (bsm-61755R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with PHOX2B monoclonal antibody, unconjugated (bsm-61755R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Human

Predicted Reactivity

Mouse

Overview
Catalog #	bsm-61755r-TR
Product Name	PHOX2B Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PHOX2B
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8929
Swiss Prot	Q99453
Subcellular location	Nucleus
Synonyms	NBLST2; NBPhox; PHOX 2B; PHOX2B; PMX 2B; PMX2B.
Background	Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100