PU.1/Spi1 Recombinant Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61756r-Cy5.5-100uL |
| Product Name | PU.1/Spi1 Recombinant Antibody, Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPI1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6688 |
| Swiss Prot | P17947 |
| Subcellular location | Nucleus |
| Synonyms | oncogene spi1; PU.1; SFPI1; SPI 1; SPI A; Spi1. |
| Background | Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is PU.1/Spi1 Recombinant Antibody, Cy5.5 Conjugated (BSM-61756R-CY5-5) validated for, and at what dilution?
BSM-61756R-CY5-5 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PU.1/Spi1 Recombinant Antibody, Cy5.5 Conjugated (BSM-61756R-CY5-5) react with?
Tested reactivity: Human, Mouse.
How should BSM-61756R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PU.1/Spi1 Recombinant Antibody, Cy5.5 Conjugated (BSM-61756R-CY5-5)?
KLH conjugated synthetic peptide derived from human SPI1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-61756R-CY5-5)?
Cy5.5 on BSM-61756R-CY5-5: excitation / emission 675nm/694nm.