Prostate Secretory Protein/PSP Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with Prostate Secretory Protein\/PSP monoclonal antibody, unconjugated (bsm-61761R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Prostate Secretory Protein\/PSP monoclonal antibody, unconjugated (bsm-61761R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)
IP

Reactivity

Human

Overview
Catalog #	bsm-61761r-TR
Product Name	Prostate Secretory Protein/PSP Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human beta 2 Microseminoprotein
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4477
Swiss Prot	P08118
Subcellular location	Secreted
Synonyms	MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94.
Background	The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer.
Application Dilution
WB	1:500-2000
IHC-P	IHC-P1:200-1000
IHC-F	IHC-F1:200-1000
IF(IHC-P)	IFIHC-P1:200-1000
IP	IP1:20-50