E2F4 Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61764r-bf405 |
| Product Name | E2F4 Recombinant Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human E2F4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1874 |
| Swiss Prot | Q16254 |
| Subcellular location | Nucleus |
| Synonyms | E2F 4; E2F transcription factor 4; E2F transcription factor 4 p107/p130 binding; E2F4. |
| Background | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is E2F4 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61764R-BF405) validated for, and at what dilution?
bsm-61764R-BF405 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does E2F4 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61764R-BF405) react with?
Tested reactivity: Human.
How should bsm-61764R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise E2F4 Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61764R-BF405)?
KLH conjugated synthetic peptide derived from human E2F4
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-61764R-BF405)?
AbBy Fluor-405 on bsm-61764R-BF405: excitation / emission 401nm/421nm.