LIMK1 Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61772r-bf647-100uL |
| Product Name | LIMK1 Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LIMK 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3984 |
| Swiss Prot | P53667 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | LIM kinase; LIMK 1; LIMK. |
| Background | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is LIMK1 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61772R-BF647) validated for, and at what dilution?
bsm-61772R-BF647 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LIMK1 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61772R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-61772R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LIMK1 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61772R-BF647)?
KLH conjugated synthetic peptide derived from human LIMK 1
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61772R-BF647)?
AbBy Fluor-647 on bsm-61772R-BF647: excitation / emission 650nm/665nm.