LIMK1 Recombinant Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61772r-pe-Cy5.5-100uL |
| Product Name | LIMK1 Recombinant Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LIMK 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3984 |
| Swiss Prot | P53667 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | LIM kinase; LIMK 1; LIMK. |
| Background | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is LIMK1 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61772R-PE-CY5-5) validated for, and at what dilution?
BSM-61772R-PE-CY5-5 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LIMK1 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61772R-PE-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should BSM-61772R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LIMK1 Recombinant Antibody, PE-Cy5.5 Conjugated (BSM-61772R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human LIMK 1
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-61772R-PE-CY5-5)?
PE-Cy5.5 on BSM-61772R-PE-CY5-5: excitation / emission 488nm/694nm.