Creatine kinase B type Recombinant Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61776r-percp-100uL |
| Product Name | Creatine kinase B type Recombinant Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Creatine kinase B type |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1152 |
| Swiss Prot | P12277 |
| Subcellular location | Cytoplasm |
| Synonyms | BCK; Ckb; CKBB; HEL 211; HEL S 29. |
| Background | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Creatine kinase B type Recombinant Antibody, PerCP Conjugated (bsm-61776R-PerCP) validated for, and at what dilution?
bsm-61776R-PerCP is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Creatine kinase B type Recombinant Antibody, PerCP Conjugated (bsm-61776R-PerCP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61776R-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Creatine kinase B type Recombinant Antibody, PerCP Conjugated (bsm-61776R-PerCP)?
KLH conjugated synthetic peptide derived from human Creatine kinase B type
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61776R-PerCP)?
PerCP on bsm-61776R-PerCP: excitation / emission 482nm/677nm.