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KDM5A Recombinant Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
Overview
Catalog # bsm-61779r-cy7-100uL
Product Name KDM5A Recombinant Antibody, Cy7 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human
Predicted Reactivity Mouse
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human KDM5A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5927
Swiss Prot P29375
Subcellular location Nucleus
Synonyms JARID1A; Kdm5a; RBBP2; RBP2.
Background Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is KDM5A Recombinant Antibody, Cy7 Conjugated (bsm-61779R-Cy7) validated for, and at what dilution?

bsm-61779R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KDM5A Recombinant Antibody, Cy7 Conjugated (bsm-61779R-Cy7) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.

How should bsm-61779R-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise KDM5A Recombinant Antibody, Cy7 Conjugated (bsm-61779R-Cy7)?

KLH conjugated synthetic peptide derived from human KDM5A

What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-61779R-Cy7)?

Cy7 on bsm-61779R-Cy7: excitation / emission 743nm/767nm.