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DNTT Recombinant Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-61808r-percp-100uL
Product Name DNTT Recombinant Antibody, PerCP Conjugated
Applications
WB IF(IHC-P)
Reactivity Human
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DNTT
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1791
Swiss Prot P04053
Subcellular location Nucleus
Synonyms DNA nucleotidylexotransferase; DNTT; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase.
Background Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Application Dilution
WB 1:500-2000
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is DNTT Recombinant Antibody, PerCP Conjugated (bsm-61808R-PerCP) validated for, and at what dilution?

bsm-61808R-PerCP is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DNTT Recombinant Antibody, PerCP Conjugated (bsm-61808R-PerCP) react with?

Tested reactivity: Human.

How should bsm-61808R-PerCP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DNTT Recombinant Antibody, PerCP Conjugated (bsm-61808R-PerCP)?

KLH conjugated synthetic peptide derived from human DNTT

What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61808R-PerCP)?

PerCP on bsm-61808R-PerCP: excitation / emission 482nm/677nm.