RING2 Recombinant Antibody, Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61821r-cy7-100uL |
| Product Name | RING2 Recombinant Antibody, Cy7 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RING2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6045 |
| Swiss Prot | Q99496 |
| Subcellular location | Nucleus |
| Synonyms | BAP1; DING; BAP-1; HIPI3; RING2; RING1B. |
| Background | Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes.Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is RING2 Recombinant Antibody, Cy7 Conjugated (bsm-61821R-Cy7) validated for, and at what dilution?
bsm-61821R-Cy7 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RING2 Recombinant Antibody, Cy7 Conjugated (bsm-61821R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61821R-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RING2 Recombinant Antibody, Cy7 Conjugated (bsm-61821R-Cy7)?
KLH conjugated synthetic peptide derived from human RING2
What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-61821R-Cy7)?
Cy7 on bsm-61821R-Cy7: excitation / emission 743nm/767nm.