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DCP1A Recombinant Antibody, FITC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bsm-61822r-fitc-100uL
Product Name DCP1A Recombinant Antibody, FITC Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DCP1A
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55802
Swiss Prot Q9NPI6
Subcellular location Cytoplasm
Synonyms Dcp1a; HSA275986; Nbla00360; SMAD4IP1; SMIF.
Background Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay.Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP.Contributes to the transactivation of target genes after stimulation by TGFB1.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is DCP1A Recombinant Antibody, FITC Conjugated (bsm-61822R-FITC) validated for, and at what dilution?

bsm-61822R-FITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DCP1A Recombinant Antibody, FITC Conjugated (bsm-61822R-FITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bsm-61822R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DCP1A Recombinant Antibody, FITC Conjugated (bsm-61822R-FITC)?

KLH conjugated synthetic peptide derived from human DCP1A

What are the excitation and emission wavelengths of the FITC conjugate (bsm-61822R-FITC)?

FITC on bsm-61822R-FITC: excitation / emission 494nm/518nm.