PSGL-1/CD162 Recombinant Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61840r-percp |
| Product Name | PSGL-1/CD162 Recombinant Antibody, PerCP Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PSGL 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6404 |
| Swiss Prot | Q14242 |
| Subcellular location | Cell membrane |
| Synonyms | CD162; CLA; PSGL1; SELPLG. |
| Background | A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture. |
| Application Dilution | |
| FCM | 1ug/Test |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is PSGL-1/CD162 Recombinant Antibody, PerCP Conjugated (bsm-61840R-PerCP) validated for, and at what dilution?
bsm-61840R-PerCP is validated for FCM(FCM) (1ug/Test); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PSGL-1/CD162 Recombinant Antibody, PerCP Conjugated (bsm-61840R-PerCP) react with?
Tested reactivity: Human.
How should bsm-61840R-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PSGL-1/CD162 Recombinant Antibody, PerCP Conjugated (bsm-61840R-PerCP)?
KLH conjugated synthetic peptide derived from human PSGL 1
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61840R-PerCP)?
PerCP on bsm-61840R-PerCP: excitation / emission 482nm/677nm.