GIT1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61866r-cy3-100uL |
| Product Name | GIT1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GIT1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 28964 |
| Swiss Prot | Q9Y2X7 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ARF GAP GIT1; CAT 1; CaT1; GIT1. |
| Background | GTPase-activating protein for ADP ribosylation factor family members, including ARF1. Multidomain scaffold protein that interacts with numerous proteins and therefore participates in many cellular functions, including receptor internalization, focal adhesion remodeling, and signaling by both G protein-coupled receptors and tyrosine kinase receptors.Through PAK1 activation, positively regulates microtubule nucleation during interphase. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is GIT1 Recombinant Antibody, Cy3 Conjugated (bsm-61866R-Cy3) validated for, and at what dilution?
bsm-61866R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GIT1 Recombinant Antibody, Cy3 Conjugated (bsm-61866R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61866R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GIT1 Recombinant Antibody, Cy3 Conjugated (bsm-61866R-Cy3)?
KLH conjugated synthetic peptide derived from human GIT1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61866R-Cy3)?
Cy3 on bsm-61866R-Cy3: excitation / emission 512,550nm/570,615nm.