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DKC1 Recombinant Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61870r-bf555
Product Name DKC1 Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Dyskerin
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1736
Swiss Prot O60832
Subcellular location Cytoplasm, Nucleus
Synonyms DKC1; DKC; CBF5; DKCX; NAP57; NOLA4; XAP101.
Background Isoform 1: Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is DKC1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61870R-BF555) validated for, and at what dilution?

bsm-61870R-BF555 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DKC1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61870R-BF555) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61870R-BF555 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DKC1 Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-61870R-BF555)?

KLH conjugated synthetic peptide derived from human Dyskerin

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-61870R-BF555)?

AbBy Fluor-555 on bsm-61870R-BF555: excitation / emission 553nm/568nm.