eIF4A1 Recombinant Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61878r-bf750 |
| Product Name | eIF4A1 Recombinant Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF4A1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1973 |
| Swiss Prot | P60842 |
| Subcellular location | Extracellular, Cytoplasm |
| Synonyms | DDX2; DDX2A; eIF 4A I; EIF4A; eIF4A I; eIF4A-I; EIF4A1. |
| Background | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is eIF4A1 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61878R-BF750) validated for, and at what dilution?
bsm-61878R-BF750 is validated for WB(WB) (1:500-2000); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF4A1 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61878R-BF750) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bsm-61878R-BF750 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF4A1 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61878R-BF750)?
KLH conjugated synthetic peptide derived from human eIF4A1
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-61878R-BF750)?
AbBy Fluor-750 on bsm-61878R-BF750: excitation / emission 749nm/775nm.