eIF4A3 Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61883r-bf647 |
| Product Name | eIF4A3 Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF4A3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9775 |
| Swiss Prot | P38919 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | DDX48; EIF4A3; eIF4AIII; hNMP 265; NMP 265; NMP265; NUK34. |
| Background | ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. |
| Application Dilution | |
| IF(IHC-P) | 1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is eIF4A3 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61883R-BF647) validated for, and at what dilution?
bsm-61883R-BF647 is validated for IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF4A3 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61883R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61883R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF4A3 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61883R-BF647)?
KLH conjugated synthetic peptide derived from human eIF4A3
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61883R-BF647)?
AbBy Fluor-647 on bsm-61883R-BF647: excitation / emission 650nm/665nm.