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eIF4A3 Recombinant Antibody, AbBy Fluor-750 Conjugated

Applications

  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61883r-bf750
Product Name eIF4A3 Recombinant Antibody, AbBy Fluor-750 Conjugated
Applications
IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF4A3
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9775
Swiss Prot P38919
Subcellular location Cytoplasm, Nucleus
Synonyms DDX48; EIF4A3; eIF4AIII; hNMP 265; NMP 265; NMP265; NUK34.
Background ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
Application Dilution
IF(IHC-P) 1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is eIF4A3 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61883R-BF750) validated for, and at what dilution?

bsm-61883R-BF750 is validated for IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does eIF4A3 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61883R-BF750) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61883R-BF750 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise eIF4A3 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61883R-BF750)?

KLH conjugated synthetic peptide derived from human eIF4A3

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-61883R-BF750)?

AbBy Fluor-750 on bsm-61883R-BF750: excitation / emission 749nm/775nm.