NUF2 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with NUF2 monoclonal antibody, unconjugated (bsm-61893R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with NUF2 monoclonal antibody, unconjugated (bsm-61893R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
Flow-Cyt
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-61893r
Product Name	NUF2 Recombinant Antibody
Applications	WB, Flow-Cyt, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NUF2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	83540
Swiss Prot	Q9BZD4
Subcellular location	Nucleus
Synonyms	CDCA1; CT106; hNuf2; hNuf2R; hsNuf2; NUF2; NUF2R.
Background	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.equired for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Application Dilution
WB	1:300-5000
Flow-Cyt	1ug/Test
ICC/IF	1:50-100