COREST Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61897r-cy3-100uL |
| Product Name | COREST Recombinant Antibody, Cy3 Conjugated |
| Applications |
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human REST corepressor 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23186 |
| Swiss Prot | Q9UKL0 |
| Subcellular location | Nucleus |
| Synonyms | COREST; Nr2b2; RCOR; Rcor1; Rxrb. |
| Background | Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is COREST Recombinant Antibody, Cy3 Conjugated (bsm-61897R-Cy3) validated for, and at what dilution?
bsm-61897R-Cy3 is validated for IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does COREST Recombinant Antibody, Cy3 Conjugated (bsm-61897R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61897R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise COREST Recombinant Antibody, Cy3 Conjugated (bsm-61897R-Cy3)?
KLH conjugated synthetic peptide derived from human REST corepressor 1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61897R-Cy3)?
Cy3 on bsm-61897R-Cy3: excitation / emission 512,550nm/570,615nm.