UCP1/3 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61899r-cy5-100uL |
| Product Name | UCP1/3 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse UCP1/3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22227 |
| Swiss Prot | P12242,P56501 |
| Subcellular location | Cytoplasm |
| Synonyms | UCP1; UCP3. |
| Background | Mitochondrial transporter that functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is UCP1/3 Recombinant Antibody, Cy5 Conjugated (bsm-61899R-Cy5) validated for, and at what dilution?
bsm-61899R-Cy5 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UCP1/3 Recombinant Antibody, Cy5 Conjugated (bsm-61899R-Cy5) react with?
Tested reactivity: Mouse, Rat.
How should bsm-61899R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UCP1/3 Recombinant Antibody, Cy5 Conjugated (bsm-61899R-Cy5)?
KLH conjugated synthetic peptide derived from mouse UCP1/3
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61899R-Cy5)?
Cy5 on bsm-61899R-Cy5: excitation / emission 625,650nm/670nm.