HIF1AN Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61905r-bf647-100uL |
| Product Name | HIF1AN Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIF 1 alpha inhibitor |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55662 |
| Swiss Prot | Q9NWT6 |
| Subcellular location | Nucleus |
| Synonyms | FIH 1; FIH1; HIF1AN. |
| Background | Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1ug/test |
| IF(IHC-P) | IFIHC-P1:200-500 |
| IF(ICC) | IFICC1:100-200 |
Frequently asked questions
Which applications is HIF1AN Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61905R-BF647) validated for, and at what dilution?
bsm-61905R-BF647 is validated for WB(WB) (1:500-2000); FCM(FCM) (1ug/test); IF(IHC-P)(IF(IHC-P)) (1:200-500); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIF1AN Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61905R-BF647) react with?
Tested reactivity: Human.
How should bsm-61905R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIF1AN Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61905R-BF647)?
KLH conjugated synthetic peptide derived from human HIF 1 alpha inhibitor
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61905R-BF647)?
AbBy Fluor-647 on bsm-61905R-BF647: excitation / emission 650nm/665nm.