HIF1AN Recombinant Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61905r-biotin |
| Product Name | HIF1AN Recombinant Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIF 1 alpha inhibitor |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 55662 |
| Swiss Prot | Q9NWT6 |
| Subcellular location | Nucleus |
| Synonyms | FIH 1; FIH1; HIF1AN. |
| Background | Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | IHC-P1:200-500 |
| IHC-F | IHC-F1:200-500 |