RHEB Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61909r-cy3-100uL |
| Product Name | RHEB Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RHEB |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6009 |
| Swiss Prot | Q15382 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | RHEB 2; Rheb; RHEB2. |
| Background | Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is RHEB Recombinant Antibody, Cy3 Conjugated (bsm-61909R-Cy3) validated for, and at what dilution?
bsm-61909R-Cy3 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RHEB Recombinant Antibody, Cy3 Conjugated (bsm-61909R-Cy3) react with?
Tested reactivity: Human.
How should bsm-61909R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RHEB Recombinant Antibody, Cy3 Conjugated (bsm-61909R-Cy3)?
KLH conjugated synthetic peptide derived from human RHEB
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61909R-Cy3)?
Cy3 on bsm-61909R-Cy3: excitation / emission 512,550nm/570,615nm.