MIB1 Recombinant Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61914r-apc-Cy5.5-100uL |
| Product Name | MIB1 Recombinant Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MIB1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57534 |
| Swiss Prot | Q86YT6 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Dip1; LVNC7; MIB; mib1; ZZANK2; ZZZ6. |
| Background | E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is MIB1 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-61914R-APC-CY5-5) validated for, and at what dilution?
BSM-61914R-APC-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MIB1 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-61914R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse.
How should BSM-61914R-APC-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MIB1 Recombinant Antibody, APC-Cy5.5 Conjugated (BSM-61914R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human MIB1
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BSM-61914R-APC-CY5-5)?
APC-Cy5.5 on BSM-61914R-APC-CY5-5: excitation / emission 650nm/694nm.