Pumilio 1 Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61918r-bf680-100uL |
| Product Name | Pumilio 1 Recombinant Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Pumilio 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9698 |
| Swiss Prot | Q14671 |
| Subcellular location | Cytoplasm |
| Synonyms | HsPUM; PUM L1; Pum1; PUMH 1; PUMH; PUMH1; Pumilio1; PUML1. |
| Background | Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE).Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Pumilio 1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61918R-BF680) validated for, and at what dilution?
bsm-61918R-BF680 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Pumilio 1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61918R-BF680) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61918R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Pumilio 1 Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61918R-BF680)?
KLH conjugated synthetic peptide derived from human Pumilio 1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-61918R-BF680)?
AbBy Fluor-680 on bsm-61918R-BF680: excitation / emission 679nm/702nm.